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  • Pichia pastoris Expression Vectors

    BioGrammatics supplies a collection of Pichia expression vectors with various combinations of Pichia promoters, secretion signals and dominant drug selection markers for either secreted or intracellular expression. All vectors are designed to use a common cloning scheme compatible with Type IIS restriction enzymes or Gibson®/NEBuilder® assembly for seamless, directional cloning of your ORF(s).

    General Features

    • Targeted Genomic Integration: Stable transformants at defined genomic loci that don't require drug selection for propagation or protein expression.
    • Multiple drug selection markers: Ability to integrate multiple vectors into the same strain to increase expression or generate multi–protein complexes. Vectors can be co–transformed in a single reaction and selected on multiple drugs.
    • Selection and propagation in E. coli with LB-amp medium rather than Zeocin or other expensive drugs.

    Seamless Cloning

    After digestion with Bsa I, all BioGrammatics Pichia expression vectors allow seamless, directional insertion of your ORF of interest using either isothermal assembly or flanking Type IIS restriction enzymes.



    Methanol Inducible Vectors

    Constitutive Expression Vectors

      Product Description
      pJAN–s1 extracellular expression vector, S. cerevisiae α mating factor leader, nourseothricin selection Details
      pJAZ–s1 extracellular expression vector, S. cerevisiae α mating factor leader, zeocin selection Details
      pJAH–s1 extracellular expression vector, S. cerevisiae α mating factor leader, hygromycin selection Details
      pJAG–s1 extracellular expression vector, methanol–inducible AOX1 promoter, S. cerevisiae α mating factor leader, G418 selection Details
      pJAN intracellular expression vector, nourseothricin selection Details
      pJAZ intracellular expression vector, zeocin selection Details
      pJAG intracellular expression vector, G418 selection Details
      pJAG–Display surface display expression vector, methanol–induced AOX1 promoter, G418 selection Details
      pJGG–Display surface display expression vector, constitutive GAP promoter, G418 selection Details
      pJAZ–s2 extracellular expression vector, truncated S. cerevisiae α mating factor leader, zeocin selection Details
      pJAG–s2 extracellular expression vector, truncated S. cerevisiae α mating factor leader, G418 selection Details
      pJAN–s2 extracellular expression vector, truncated S. cerevisiae α mating factor leader, nourseothricin selection Details
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