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BioGrammatics offers two series of Pichia pastoris expression strains. Our house strain series (BG strains) is derived from a wild Pichia pastoris strain originally isolated by Herman Phaff in California during the 1940's. Our single colony isolate of the Phaff strain was fully sequenced using Illumina HiSeq technology. This original BioGrammatics strain, BG08, was the starting point for our strain series. BG08 was grown under selective conditions to eliminate cytoplasmic killer plasmids and generate our wild type expression strain, BG10. With the exception of BG09, all subsequent strains in the BG series are derived by precise genomic manipulation of BG10. BG09 is a specific variant of BG08 that has been developed for isotopically labeling proteins with 13C at tryptic cleavage sites for mass spectroscopy studies.
In addition to the BG series, BioGrammatics is also a distributor of RCT's GlycoSwitch® strains. The GlycoSwitch® strain series is derived from the Pichia pastoris GS115 strain developed at Phillips Petroleum in the 1970's. GS115 is a mutagenized isolate of one of the original Phaff strains, selected for histidine auxotrophy. The original GlycoSwitch® Man5 strain originates from collaborations amongst Roland Contreras, Ph.D., Nico Callewaert, Ph.D., and their colleagues at Ghent University and the Flanders Institute for Biotechnology (VIB) in Belgium. The SuperMan5 strains are subsequent modifications of GlycoSwitch® Man5 generated by BioGrammatics, in which all regions of genomic homology at the mutant och1 locus have been removed to prevent reversion to wild type by homologous recombination.
All strains are available as YPD stabs. A selection of strains is also available as electrocompetent cells—ready for transformation.
|SuperMan5 (HIS+)||GlycoSwitch® strain, Man5 N-linked oligosaccharide structures||Details|
|SuperMan5 (his—)||GlycoSwitch® strain, Man5 N-linked oligosaccharide structures (histidine auxotroph)||Details|
|Pichia pastoris BG10 strain||Wild type Pichia pastoris expression strain (killer plasmid free)||Details|
|Pichia pastoris BG11 strain||Derivative of Pichia pastoris BG10 strain, aox1Δ, MutS (methanol utilization slow)||Details|
|SuperMan5 (HIS+, pep4Δ)||GlycoSwitch® strain, Man5 N-linked oligosaccharide structures (PEP4 protease deletion)||Details|
|Pichia pastoris BG09 strain||Derivative of Pichia pastoris BG08 strain, arg4Δ::nourseothricinR, lys2Δ::hygromycinR (arginine, lysine auxotroph)||Details|
|Pichia pastoris BG12 strain||Derivative of Pichia pastoris BG10 strain, his4Δ (histidine auxotroph)||Details|